Role of metabotropic glutamate receptors in dendritic coordination between excitation and inhibition
Coordination between excitation and inhibition is essential for proper functioning of neuronal circuits. We recently discovered that by repeated stimulation of excitatory synapses, the formation of a nearby inhibitory bouton onto the same dendrite can be triggered. We showed that NMDA receptor activation is required, but not sufficient, to induce bouton growth. Here, we hypothesized that additional activation of metabotropic glutamate receptors 5 (mGluR5s) is necessary. We used two-photon microscopy to image crossings between CA1 pyramidal cell dendrites and GABAergic axons in organotypic hippocampal slices of GAD65-GFP mice. We stimulated multiple dendritic spines close to the crossing axon using two-photon glutamate uncaging (30x @0.5Hz). Our results indicate that inhibitory bouton growth seems to be independent of mGluR5 activation.
I'm intrigued by how information processing in networks of neurons shape our behavior. To study these networks we need to understand how they are build up, relay information between and within neurons and how this processing is modulated.
For my PhD dissertation I studied the role of developmental proteins in the mature brain and in relation to febrile seizures at UMCU/UU with Profs. Pasterkamp and Burbach, Drs. Ramakers and de Graan. Here I discovered that these developmental proteins can have great influence on how a newly born neuron integrates into an adult neuronal network. Establishing that proteins essential for neurodevelopment retain important functions in the adult brain.
During my first post-doctoral fellowship in the labs of Drs. Mao and Zhong at the Vollum Institute in Portland (OR, US) I worked on a variety of projects all with the common purpose of understanding how the diversity in neuronal connectivity and identity establishes a network that is able to process information in a adaptable fa
Yaron Caspi Andrei
UU, Faculty of Science
UU, Faculty of Veterinary Medicine
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