Cell cycle dependency of CRISPR/Cas gene editing

CRISPR-Cas9 gene correction is a therapeutic tool which may abolish half of the known genetic disorders. To achieve this we need to deliver three macromolecules into the nucleus simultaneously, as well as trigger the homology-directed repair (HDR) pathway. The focus of this poster is on the role of mitosis on the state of these barriers, which in theory removes the nuclear membrane barrier and primes the cell for the HDR pathway.

The mitotic state of the cell skews the gene-editing outcome greatly, indicating a need to target mitotic cells for therapeutic gene correction.

16-11-2020 15:15 - 15:30