Interference of lipoprotein particles during flow cytometric analysis of single extracellular vesicles in plasma
Cell derived extracellular vesicles (EVs) in blood plasma have been associated with diseases, making them attractive for diagnosis and prognosis of patients. High-resolution flow cytometry (FC) allows for high throughput analysis and detection of single EVs and enables quantitative and qualitative characterization. However, plasma contains a large number of different types of lipoprotein particles (LPP) and their presence may hamper robust flow cytometric analysis of EVs due to partially overlapping biophysical properties such as size, density and molecular makeup. We therefor investigated the possible interference of LPP in single EV analysis performed by fluorescence-based FC.