Extracellular vesicles (EVs) play a pivotal role in intercellular communication through functional transfer of bioactive cargo, including RNA molecules. Despite increasing interest in EV-mediated RNA transfer, understanding of the pathways and mechanisms regulating EV-mediated RNA delivery and processing is limited due to a lack of suitable readout systems. Here, we show a novel CRISPR/Cas9-based reporter system that allows the study of EV-mediated RNA transfer at single-cell resolution. We validate this system by studying the role of known targets involved in EV uptake and intracellular membrane trafficking, and subsequently employ this system to uncover various novel genes that play a regulatory role in functional RNA transfer. Altogether, this novel approach allows the study of specific genetic targets and pathways in EV-mediated functional RNA delivery, and unravel the regulatory pathways that dictate the underlying processes.
16-11-2020 16:15 - 16:30Hosted by: Olivier de Jong
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